The RNAi vecter construction and verification of the functions in sexual control of Cervus elaphus Zfx gene

• DOI: 10.21203/rs.2.19002/v1
• Publication Date: 2019-12-17T17:19:47Z
• Authors: Limin Wei; Song Jiang; Jifeng Xi; Yongsheng Zhang; Bo Zeng; Feng Li; Kaisheng Wang; Siyuan Zhang; Xitang Zhao; Ying Li; Hong Shen; Bin Jia
• Keywords: Cervus elaphus

Abstract Background: Zinc finger protein X-linked ( Zfx ) was regarded to be a sex determination factor, and plays critical role in spermatogenesis. RNAi is an effective method of silencing / Zfy mRNA expression. However, there has been little research on the use technology control offspring Cervus elaphus . The objective this study first explore efficient alter sex-ratio by genes during Results: Three recombinant expression vectors pLL3.7/A, pLL3.7/B pLL3.7/C were constructed interrupt gene. results showed that significantly silenced pLL3.7/A P < 0.01), compared with group. group injected produced 94 , including 68 males 26 females. male rates (72.34%) higher than groups 0.01). Conclusions: Our experiment suggest gene significant process X-sperm formation. siRNA may useful approach