Notoginsenoside R1 (NGR1) induce DNA damage to inhibit cervical cancer cells proliferation by inhibiting PHF6 activity

• DOI: 10.21203/rs.2.20516/v1
• Publication Date: 2020-01-09T20:23:08Z
• Authors: Ting Cai; Wenquan Wu; Qiang Ming; Longhua Guo; Yongwu Xia; Yunhong Liu; Xiaoxin Jiang; Limei Zhang; Feiding Peng; pinghong ming
• Keywords: Panax notoginseng

Abstract Notoginsenoside R1 (NGR1) is isolated from the panax notoginseng which a kind of Traditional Chinese Medicine and edible plant with good healthful effect that using range very wide for medical treatment health care. It had been demonstrated to inhibit various tumors proliferation, but whether it inhibited cervical cancer cells proliferation its mechanism was not unclear. In this study, we showed NGR1 could proliferate time dose dependent manner, induce apoptosis arrest in G1/S-phase . We also found make H2AX phosphorylation PHF6 expression manner. Furthermore, when over gene, γH2AX haven't any change , silenced gene siRNA, increased significantly. That mean has negative correlation γH2AX. Subsequently added intervene, something interesting happened protein fell even more, more up regulation siPHF6 group. PEGFP-C1-PHF6 plasmid vector group, inversely declined, still All those results indicate d caused DNA injury by inhibiting activity pathway arrested G1/S-phase.