Seed characterization and early Nitrogen metabolism performance of seedlings from Altiplano and Coastal ecotypes of Quinoa

• DOI: 10.21203/rs.2.24363/v3
• Publication Date: 2020-07-07T18:41:08Z
• Authors: Katherine Pinto/I...; Teodoro Coba de l...; Enrique Ostria-Ga...; Cristian Ibanez; Vilbett Briones; Alexander Vergara; Rodrigo Alvarez; Catalina Castro; Carolina Sanhueza; Patricio Castro; Luisa Bascunan-Godoy
• Keywords: Ecotype; Chenopodium quinoa

Abstract Background: Early seed germination and a functional root system development during establishment are crucial attributes contributing to nutrient competence under marginal soil conditions. Chenopodium quinoa Willd (Chenopodiaceae) is rustic crop, able grow in areas. Altiplano Coastal/Lowlands two representative zones of cultivation South America with contrasting fertility edaphoclimatic conditions.In the present work, we hypothesize that ecotypes Quinoa from (landrace Socaire) Coastal/Lowland Faro) have developed differential adaptive responses order survive conditions low availability N their respective climatic Lowlands. In understand intrinsic differences for between landraces, metabolite profile germinative capacity were studied. Additionally, elucidate mechanisms uptake assimilation at limiting establishment, germinated seeds both landraces grown either sufficient nitrate (HN) or (LN) supply. We studied photosynthetic performance, protein storage, morphometrical parameters, activity expression N-assimilating enzymes, transporters roots plants submitted different treatments. Results: Seeds Socaire landrace presented higher content free N-related metabolites faster rate compared Faro landrace. Seedlings similar physiological performance HN supply, but LN supply exalted. At LN, showed an increased biomass (including number total length lateral roots), regulation transporter (a NPF6.3 -like homologue) belonging Low Affinity Transport System (LATS), upregulation NRT2.1 High (HATS) Faro. These as whole could be linked amount stored proteins leaves, associated enhanced photochemical plants, comparison Lowland plants. Conclusions: characteristics over involve adaptation brutal unfavorable climate Altiplano.