Genetic diversity and genotype multiplicity of Plasmodium falciparum infection in patients with uncomplicated malaria in Chewaka district, Ethiopia

• DOI: 10.21203/rs.2.24526/v3
• Publication Date: 2020-05-29T17:24:53Z
• Authors: Abdulhakim Abamecha; Hassan El-Abid; Daniel Yilma; Wondimagegn Addisu; Achim Ibenthal; Abebe Genetu Bayih; Harald Noedl; Delenasaw Yewhalaw; Mohieddine Moumni; Alemseged Abdissa
• Keywords: Merozoite surface protein; Multiplicity of infection; Artemether/lumefantrine

Abstract Background Genetic diversity in Plasmodium falciparum poses a major threat to malaria control and elimination interventions. Characterization of the genetic P. strains can be used assess intensity parasite transmission identify potential deficiencies programmes, which provides vital information evaluating efforts. This study investigated genotype multiplicity infection isolates from cases with uncomplicated Southwest Ethiopia. Methods A total 80 microscopy qPCR positive blood samples were collected participants aged six months sixty years, who visited health facilities during efficacy artemether-lumefantrine September-December, 2017. Polymorphic regions msp-1 msp-2 genotyped by nested polymerase chain reactions (nPCR) followed gel electrophoresis for fragment analysis. Results Of qPCR-positive analysed polymorphisms on genes, efficiency gene amplification family-specific primers 95 % 98.8%, respectively. Allelic variation 90% (72/80) 86.2% (69/80) observed. K1 was predominant allelic family detected 20.8% (15/72) MAD20 RO33. Within , FC27 showed higher frequency (26.1%) compared IC/3D7 (15.9%). Ten different alleles observed 6 3 1 allele In 19 individual 10 9 3D7. Eighty percent (80%) had multiple genotypes overall mean 3.2 (95% CI: 2.87- 3.46). The heterozygosity indices 0.43 0.85 There no significant association between age or density. Conclusions revealed high levels mixed-strain infections populations Chewaka district, Ethiopia, suggesting that both endemicity level remain strengthened efforts are needed