Somatic Embryogenesis in Tissue Cultures of Pecan

• DOI: 10.21273/hortsci.22.1.128
• Publication Date: 2022-09-15T16:42:51Z
• Authors: Scott A. Merkle; Hazel Y. Wetzstein; Harry E. Sommer
• Keywords: Juglandaceae

Abstract Tissue cultures of pecan [ Carya illinoensis (Wangenh.) C. Koch] were initiated from embryo explants collected weekly 12 weeks post-pollination until fruit maturity. Three derived immature embryos 14 postpollination produced primary somatic within 1 month following transfer modified woody plant medium (WPM) with 2.0 mgliter −1 2,4-D and 0.25 mg liter BA in the light to hormone-free dark. Scanning electron microscopy documented development secondary embryos, which followed a globular, heartshaped, torpedo-stage developmental sequence. Chemical names used: (2,4-di-chlorophenoxy) acetic acid (2,4-D), N -(phenylmethyl)-1 H -purin-6-amine (BA), H- indole-3-butanoic (IBA).