Cytotoxic Effect of PEI-Coated Magnetic Nanoparticles on the Regulation of Cellular Focal Adhesions and Actin Stress Fibres

• DOI: 10.2139/ssrn.3558242
• Publication Date: 2020-03-26T11:31:20Z
• Authors: Kaarjel K. Naraya...; Joshua C. Price; Marwan Merkhan; Ajile Elttayef; Jon Dobson; Neil D. Telling
• Keywords: 0303 health sciences; 03 medical and health sciences; QR

The biocompatibility of surface-coated magnetic nanoparticles (MNPs) is key to their successful use in biomedical applications. Polyethyleneimine-coated MNPs (MNP-PEIs) provide improved vitro nucleic acid transfection efficiency and are safer compared conventional chemicals. Commercial cell toxicity assays useful for end-point high-throughput screening, however, they only reports cells that have undergone an extreme toxic response leading death. Cell a complex process which can be expressed many forms, through morphological, metabolic, epigenetic changes. This study explores the effect with MNP-PEIs external field on toxicity, by studying particle internalization, changes cellular morphology, adhesion. We show induce stress dose-dependent increase adhesion via overexpression vinculin formation actin fibres. While presence PEI was main contributor increased stress, free polyplexes induced higher bound MNPs. without coating however did not adversely affect cells, suggesting chemical instead mechanical one. In addition, genes identified as being associated fibre regulation showed significant increases expression from MNP-PEI internalization. From these results, we identify anomalous behaviour, gene after interaction MNP-PEIs, well safe dosage reduce acute toxicity.