Photodynamic Therapy Repairs Bisphosphonate-Related Osteonecrosis of the Jaw by Modulating the NF-kB Pathway in Rats

• DOI: 10.2139/ssrn.4100190
• Publication Date: 2022-05-27T23:46:16Z
• Authors: Jannerson Pontes; Ludmila Figueiredo; Wilson Lima; Maria Carolina Sousa; Rubens Araújo; Ana Beatriz Santos; Leopoldina de Alm...; Adriano Alves

This study aimed to evaluate if antimicrobial photodynamic therapy (aPDT) repairs bisphosphonate-related osteonecrosis of the jaw (BRONJ) modulated by nuclear factor - kappa B (NF-kB) pathway in a murine model. The animals were divided into groups: negative control (NC), positive (PC), experimental (ONE) + photosensitizer (PS), ONE photobiomodulation (PBM), and (aPDT). Over 8 weeks, was induced administering 250 µg/kg zoledronic acid animals, with 0.9% saline solution being injected intraperitoneally NC group. triggering for extraction lower left first molar. Laser irradiation performed under red light (wavelength ~660 nm, power 50 mW, energy 2 J, dose 66.67 J /cm2, time 40 s) 4 weeks. Methylene blue (100 µg/mL) used as PS. euthanized observed macroscopically presence/absence exposed bone epithelial repair microscopically histochemical immunohistochemical methods. macroscopic histomorphometric data analyzed means one-way analysis variance Tukey’s post-test, statistical significance level 5% (p < 0.05), establish differences among groups. In NC, ONE+PS, ONE+PBM, ONE+aPDT groups, mucosal repair, preserved osteocytes, immunostaining against NF-kB region. PC group showed no showing anucleated osteocytes multifocal NF-kB. ONE+PBM greater deposition extracellular matrix amount necrotic tissue found. It is concluded that use PBM aPDT treatment BRONJ effective addition reducing inflammation. decreased inflammation mediated irradiated regions.