Single-Cell Transcriptomics Reveals Distinct Cell Response between Acute and Chronic Pulmonary Infection of <i>Pseudomonas Aeruginosa</i>

• DOI: 10.2139/ssrn.4141243
• Publication Date: 2022-06-28T12:19:52Z
• Authors: Xueli Hu; Mingbo Wu; Teng Ma; Yige Zhang; Chaoyu Zou; Ruihuan Wang; Yongxin Zhang; Yuan Ren; Qianqian Li; Huan Liu; Heyue Li; Taolin Li; Xiaolong Sun; Yang Yang; Miao Tang; Jing Li; Xiang Gao; Taiwen Li; Xikun Zhou
• Keywords: Pulmonary infection

Background: Knowledge of the changes in immune microenvironment during pulmonary bacterial acute and chronic infections is limited. The dissection systems may provide a basis for effective therapeutic strategies against infection.Methods: Using single-cell transcriptomics, mIHC flow cytometry, we revealed large-scale comprehensive cell composition high variation cell–cell interactions after Pseudomonas aeruginosa infection.Findings: Bacterial infection reprograms genetic architecture populations. We identified specific types, including Cxcl2+ B cells interstitial macrophages, response to infection, such as their proportions, distribution, functional status. distinct molecular signatures highlight importance highly dynamic interaction process different pathological conditions, which has not been completely previously.Interpretation: These findings unbiased cellular landscape respiratory pathogenesis mice, enabling further understanding immunologic mechanisms inflammatory diseases.Funding Information: This work was supported by National Natural Science Foundation China (No. 81922042, 82072999 82172285), Clinical Research Center Geriatrics, West Hospital, Sichuan University Z2018B02), Excellent Young Scientist 2017SCU04A16), Innovative Spark 2018SCUH0032), Major Scientific Technological Special Project “Significant New Drugs Development” 2018ZX09201018-013) 1·3·5 project excellent development discipline Hospital ZYYC21001).Declaration Interests: authors declare that they have no competing interests.Ethics Approval Statement: Eight-week-old female C57BL/6 mice were purchased from Dossy Experimental Animals Company housed specific-pathogen-free facility at State Key Laboratory Biotherapy, University. All animal experiments reviewed approved Ethics Committee 20140104) carried out compliance with institutional guidelines concerning use care