Establishment of a Xenograft Model of Human Retinoblastoma in Pig Eyes

• DOI: 10.2139/ssrn.4250908
• Publication Date: 2022-11-04T20:36:19Z
• Authors: Martina Kodetova; Karel Svojgr; Radka Hobzova; Jakub Sirc; Jan Hrabeta; Barbara Feriancikova; Daniela Kodetova; Jiri Uhlik; Ana-Irina Cocarta; Kusum Shrestha; Taras Ardan; Yaroslav Nemesh; Jan Motlik; Jana Cizkova; Jana Juhasova; Stefan Juhas; Zbynek Stranak; Pavel Pochop
• Keywords: Retinoblastoma

Retinoblastoma (Rb), the most common primary intraocular tumor in childhood, is specific its location inside eye globe and, more advanced disease, occurrence of vitreous seeds. Finding an effective treatment thus requires adequate animal model. As a large model has not yet been described, purpose this study was to create porcine Rb Eight male minipigs (8 eyes) without immunosuppression were used study. A suspension cultured WERI-Rb-1 cells, 100 µl with concentration 106 cells/ml, injected into subretinal space via pars plana vitrectomy (4 or directly eyes). The animals examined weekly using fundus camera, ultrasound and optical coherence tomography. After period 4 weeks, euthanized enucleated eyes histopathologically examined. cell growth establishment under retina and/or observed all after application 2 intravitreal cells. Tumor detectable fundoscopy starting at week 1 postinjection. generation also confirmed histopathologically. successful creation tumors nonimmunosuppressed pigs demonstrated. xenograft seeds can serve as useful platform for testing new modalities therapy.