Clustering Human Dental Pulp Fibroblasts Spontaneously Activate NLRP3 and AIM2 Inflammasomes and Induce IL-β Secretion

• DOI: 10.2139/ssrn.4307031
• Publication Date: 2023-01-01T10:34:25Z
• Authors: Shafei Zhai; Lihui Zhang; Xue Li; Changkui Liu; Qi Yu
• Keywords: AIM2

Objectives: The objective of the present study was to investigate whether NOD-like receptor family pyrin domain-containing 3 (NLRP3) and absent in melanoma 2 (AIM2) inflammasomes pathways were involved an experimental model fibroblast activation named nemosis, which used mimic circumstances without bacteria stimulation.Methods: Nemosis human dental pulp (DPFs) induced by three-dimensional culture U-shaped 96-well plates investigated scanning electron microscopy (SEM). DPFs monolayers as control. RNA extraction, quantitative real-time polymerase chain reaction (qRT-PCR), cytokine measurements, and, effect COX-2 inhibitor on spheroids studied.Results: SEM observed clusters cell membranes damage surface spheroids. displayed significant amounts NLRP3, AIM2, caspase-1 mRNA expression high IL-1β concentrations (P < 0.05) than monolayers. Specific (NS-398) decreased NLRP3 but had no impact AIM2 expression.Conclusions: In conclusion, clustering spontaneously activated secretion could be partially attenuated inhibitor. Thus, nemosis become a powerful for studying mechanisms underlying aseptic pulpitis.