Cleaning Up Western Blot Signals From Immunoprecipitated Samples Using Alternative Detection Methods

• DOI: 10.2144/000114149
• Publication Date: 2016-07-06T04:29:53Z
• Authors: Deborah L. Grainger; Ban Hudong; Du Chang; Ping Lan
• Keywords: Immunoprecipitation; Protein detection

High background signal is a common problem experienced when detecting proteins isolated through immunoprecipitation (IP) by Western blotting (WB). The most frequent cause of high interference from the heavy and light chain fragments denatured immunoprecipitating (or capture) antibody ' by-products IP labeled species-specific secondary antibodies at WB stage. Here we comment on alternative methods for detection immunoprecipitated that avoid labeling to varying extents. Certain have been described elsewhere (1), however, their use remains less widespread than traditional despite offering researcher considerable advantages.