Novel Vectors for Co-Expression of Two Proteins in E. coli

• DOI: 10.2144/01312st03
• Publication Date: 2018-09-05T16:19:03Z
• Authors: Natalya Kholod; Tomas Mustelin
• Keywords: 0303 health sciences; 03 medical and health sciences; QH301-705.5; Biology (General)

Two new vectors, pAC28 and pEGST, for the co-expression of recombinant genes in E. coli were developed. This two-plasmid system allows for an efficient expression and purification of large amounts of protein-protein complexes formed in bacterial cells. We have utilized this new system to express and isolate a stable complex of two human proteins, hematopoietic cell tyrosine phosphatase (HePTP) and mitogen-activated proteins kinase Erk2. This approach is useful for biochemical and structural studies of protein-protein interactions.