A New Approach to Epithelial–Mesenchymal Transition Diagnostics in Epithelial Tumors: Double Immunofluorescent Staining and Flow Cytometry

• DOI: 10.2144/btn-2020-0024
• Publication Date: 2020-08-11T08:19:48Z
• Authors: Tatiana A Bogush; Anna A Basharina; Baina K Eliseeva; Sergey A Kaliuzhny; Elena A Bogush; Vladislav Y Kirsanov; Mikhail M Davydov; Vyacheslav S Koso...
• Keywords: Epithelial-Mesenchymal Transition; QH301-705.5; Fluorescent Antibody Technique; Kaplan-Meier Estimate; epithelial–mesenchymal transition; 03 medical and health sciences; Biomarkers, Tumor; Humans; prognostic value; Neoplasms, Glandular and Epithelial; Biology (General); immunofluorescent assay; Proportional Hazards Models; Ovarian Neoplasms; 0303 health sciences; flow cytometry; cytokeratins; Flow Cytometry; Progression-Free Survival; 3. Good health; Gene Expression Regulation, Neoplastic; ovarian cancer; Keratins; Female; Single-Cell Analysis

A new method of double immunofluorescent staining for flow cytometry has been created to evaluate quantitative expression of mesenchymal protein vimentin only in epithelial cells of a solid tumor that is a mix of different origin cells. De novo vimentin expression is strongly associated with epithelial-mesenchymal transition and therefore is a metastatic potential marker of epithelial tumor cells. In comparison with semiquantitative available methods, the proposed one has several advantages, such as the accurate measurement of the marker's expression, and minimization of spatial and temporal tumor heterogeneity. Clinical validation of the method has revealed inverse correlation between the quantitative index of epithelial-mesenchymal transition level and progression-free survival using Kaplan-Meier curves and the COX proportional hazards ratio in 32 ovarian cancer patients.