Andrographolide potentiates the antitumor effect of topotecan in acute myeloid leukemia cells through an intrinsic apoptotic pathway

• DOI: 10.2147/cmar.s160924
• Publication Date: 2018-05-09T20:24:23Z
• Authors: Mohammad Hassan H...; Achraf Jardaly; Sarah abi Raad; Annalise Zouein; Sandra Rizk
• Keywords: Andrographolide; Propidium iodide; Andrographis Paniculata; U937 cell; Topotecan

Topotecan (TP) is an anticancer drug acting as topoisomerase I inhibitor that used in the treatment of many types cancers including leukemia, but it has significant side effects. Andrographolide, a compound extracted from Andrographis paniculata, was recently proven to inhibit growth cancer cells and can induce apoptosis. The aim this study investigate possible synergism between TP andrographolide acute myeloid vitro.U937 leukemic were cultured using Roswell Park Memorial Institute (RPMI) medium then treated for 24 h with prepared through dilution dimethyl sulfoxide (DMSO) stocks RPMI on day treatment. Cell proliferation assessed cell assay upon both compounds separately combination. Cell-cycle apoptosis detection performed by staining propidium iodide (PI) stain Annexin V/PI stain, respectively, followed flow cytometry analysis. Western blotting assess expression various proteins involved apoptotic pathways.Both showed antiproliferative effect dose-dependent manner when applied U937 separately; however, pretreating before applying exhibited synergistic lower inhibitory concentrations (half-maximal concentration). Treating alone led specific cell-cycle arrest at S phase more prominent pretreatment combination andrographolide. Using proapoptotic following increase number cells, which supported blot results manifested upregulation several expression.The low doses enhancement inducing compared application each separately.