NKAP functions as an oncogene and its expression is induced by CoCl₂ treatment in breast cancer via AKT/mTOR signaling pathway

• DOI: 10.2147/cmar.s178919
• Publication Date: 2018-10-28T18:23:41Z
• Authors: Jiangtao Liu; Honghui Wang; Yanhai Yin; Qing Li; Mei Zhang
• Keywords: 0301 basic medicine; 03 medical and health sciences; breast cancer; NKAP; Cancer Management and Research; hypoxia; Neoplasms. Tumors. Oncology. Including cancer and carcinogens; RC254-282; AKT/mTOR signaling pathway; Original Research; 3. Good health

Purpose: NKAP plays an important role in transcriptional repression, T-cell development, maturation and function acquisition, maintenance survival of hematopoietic stem cells, RNA splicing. In this study, we tried to explore the physiological breast cancer. Methods: We investigated expression cancer patients normal controls its correlation with by searching on GEPIA. knocked down MCF-7 cells RNAi technique studied effect cell proliferation, migration, invasion, apoptosis. And revealed under hypoxic conditions vitro. Results: was differentially expressed tissues is a potential prognostic indicator Subsequently, knockdown significantly inhibited proliferation clonality induced apoptosis through caspase 3-dependent pathway. addition, could strongly inhibit migration invasion cells. affected AKT/mTOR signaling pathway markedly reduced phosphorylation AKT mTOR, as well downstream protein. What's interesting CoCl 2 found induce Downregulation hindered impact including adjusting signaling. Conclusion: functioned oncogene, hypoxia via Keywords: NKAP, cancer, hypoxia,