<p>Acid-responsive nanoparticles as a novel oxidative stress-inducing anticancer therapeutic agent for colon cancer</p>

• DOI: 10.2147/ijn.s189923
• Publication Date: 2019-02-27T23:25:47Z
• Authors: Chengwei Zhao; Weilan Cao; Hailun Zheng; Zhongxiang Xiao; Jie Hu; Lehe Yang; Min Chen; Guang Liang; Suqing Zheng; Chengguang Zhao
• Keywords: Camptothecin; Viability assay

Objective: Nanoparticles can efficiently carry and deliver anticancer agents to tumor sites. Mounting evidence indicates that many types of cancer cells, including colon cancer, have a weakly acidic microenvironment increased levels reactive oxygen species. The construction nano drug delivery vehicles "activatable" in response the is new antitumor therapeutic strategy. Methods: Cinnamaldehyde (CA) was designed link directly with dextran form polymer through an acid cleavable acetal bond. Herein, novel pH-sensitive system constructed co-encapsulated 10-hydroxy camptothecin (HCPT). Dynamic light scattering (DLS) analysis, transmission electron microscopy (TEM) release kinetics analysis HCPT-CA-loaded nanoparticles (PCH) were conducted investigate physical chemical properties. cellular uptake signatures observed by confocal flow cytometry. Cell viability, cell scratch assay, apoptosis colony formation assay performed examine potent antiproliferative apoptotic effects PCH. mechanism treatment PCH evaluated Western blotting, cytometry, TEM analysis. pharmacokinetics examined healthy Sprague Dawley rats within 6 hours after sublingual vein injection. We lastly biodistribution vivo activity using xenograft mouse models HCT116 cells. Results: Both HCPT CA quickly released microenvironment. not only induced death generation intracellular species vitro but also facilitated uptake, effectively prolonged circulation, accumulation More attractively, exhibited excellent performance better systemic safety. Conclusion: Overall, utilized control release, improve pharmacokinetics, passively target tissue, exerted synergistic effect. acid-responsive has enormous potential as Keywords: cinnamaldehyde, hydroxy camptothecin, ROS, pH-responsive nanoparticles,