Permeation of antigen protein-conjugated nanoparticles and live bacteria through microneedle-treated mouse skin

• DOI: 10.2147/ijn.s20413
• Publication Date: 2011-06-21T04:43:26Z
• Authors: Cui; Amit Kumar; Cui; Xinran Li; Michael A Sandoval; Leticia B Rodriguez; Brian R Sloat
• Keywords: Medicine (General); Ovalbumin; Colony Count, Microbial; Dose-Response Relationship, Immunologic; Nanoconjugates; Administration, Cutaneous; Permeability; Mice; 03 medical and health sciences; R5-920; Drug Delivery Systems; 0302 clinical medicine; International Journal of Nanomedicine; Escherichia coli; Animals; Antigens; Particle Size; Original Research; Skin; 3. Good health; Mice, Inbred C57BL; Needles; Immunoglobulin G; Microtechnology; Female; Immunization; Porosity

Background: The present study was designed to evaluate the extent which pretreatment with microneedles can enhance skin permeation of nanoparticles in vitro and vivo. Permeation live bacteria, are physically or microparticles, through mouse pretreated also studied potential risk microbial infection. Methods results: It found that allowed solid lipid nanoparticles, size 230 nm, ovalbumin conjugated on their surface. Transcutaneous immunization a area induced stronger antiovalbumin antibody response than using alone. dose antigen determined whether microneedle-mediated transcutaneous immune subcutaneous injection same nanoparticles. Microneedle treatment permitted Escherichia coli , but not greater enabled by hypodermic injection. Conclusion: microneedle-treated antigens carried potentially induce strong response, bacterial infection associated microneedle is no Keywords: responses, safety microneedles, transepidermal water loss