p53 Promoted Ferroptosis in Ovarian Cancer Cells Treated with Human Serum Incubated-Superparamagnetic Iron Oxides

• DOI: 10.2147/ijn.s282489
• Publication Date: 2021-01-12T11:58:38Z
• Authors: Yunhan Zhang; Meihui Xia; Zizhen Zhou; Xiaoqing Hu; Jiabin Wang; Meiyu Zhang; Yi Li; Liankun Sun; Fangfang Chen; Huimei Yu
• Keywords: Viability assay; GPX4

In this study, we used MTT assays to demonstrate that a combination of SPIO-Serum and wild-type p53 overexpression can reduce ovarian cancer cell viability in vitro. Prussian blue staining iron were determine changes intracellular concentration following treatment. TEM was evaluate any mitochondrial damage induced by treatment, Western blot the expression transporter lipid peroxidation regulator proteins. JC-1 measure membrane potential, ROS levels estimated flow cytometry. Finally, xCT protein confirmed using fluorescence microscopy.SPIO-Serum effectively generated abundant toxic ROS. It also facilitated downregulation GPX4 xCT, ultimately resulting iron-dependent oxidative death. These effects could be reversed chelator DFO inhibitor Fer-1. treatment disrupted homeostasis regulating uptake cells presented with missing cristae ruptured outer membranes. Moreover, able show contributed SPIO-Serum-induced ferroptosis cells.SPIO-Serum overexpressed cells. Our data provide theoretical basis for as novel death phenotype nanomaterials.