Nanohydroxyapatite (nHAp) Doped with Iron Oxide Nanoparticles (IO), miR-21 and miR-124 Under Magnetic Field Conditions Modulates Osteoblast Viability, Reduces Inflammation and Inhibits the Growth of Osteoclast – A Novel Concept for Osteoporosis Treatment: Part 1

• DOI: 10.2147/ijn.s303412
• Publication Date: 2021-05-18T01:34:30Z
• Authors: Krzysztof Marycz; Agnieszka Smieszek; Klaudia Marcinkowska; Mateusz Sikora; Eliza Turlej; Paulina Sobierajska; Adrian Patej; Alina Bienko; Rafal J. Wiglusz
• Keywords: Viability assay; Bone remodeling; Biomaterial

Osteoporosis results in a severe decrease the life quality of many people worldwide. The latest data shows that number osteoporotic fractures is becoming an increasing international health service problem. Therefore, new kind controllable treatment methods for extensively desired. For reason, we have manufactured and evaluated nanohydroxyapatite (nHAp)-based composite co-doped with iron oxide (IO) nanoparticles. biomaterial was used as matrix controlled delivery miR-21-5p miR-124-3p, which proven impact on bone cell metabolism.The nanocomposite Ca5(PO4)3OH/Fe3O4 (later called nHAp/IO) obtained by wet chemistry method functionalised microRNAs (nHAp/IO@miR-21/124). Its physicochemical characterization performed using XRPD, FT-IR, SEM-EDS HRTEM SAED methods. modulatory effect tested vitro murine pre-osteoblasts MC3T3-E1 pre-osteoclasts 4B12. Moreover, anti-inflammatory effects were analysed model LPS-treated macrophages RAW 264.7. We cells' viability, mitochondria membrane potential oxidative stress under magnetic field (MF+) without (MF-). supplemented RT-qPCR Western blot assays to evaluate expression profile master regulators indicated pro-osteogenic nHAp/IO@miR-21/124 enhanced exposure MF. osteogenesis guided presence associated increased metabolism progenitor cells activation osteogenic markers (Runx-2, Opn, Coll-1). Simultaneously, decreased differentiation pre-osteoclastic 4B12 accompanied reduced CaII Ctsk. Obtained regulated viability showed immunomodulatory properties inhibiting inflammatory markers, ie, TNF-α, iNOs or IL-1β, LPS-stimulated 264.7 cells.We described first time concept osteoporosis based application. fabricated might proper regeneration bone, restoring balance between osteoblasts osteoclast.