Non-Modified Ultrasound-Responsive Gas Vesicles from Microcystis with Targeted Tumor Accumulation

• DOI: 10.2147/ijn.s342614
• Publication Date: 2021-12-29T09:51:18Z
• Authors: Huan Long; Xiaojuan Qin; Rui Xu; Chunlei Mei; Zhiyong Xiong; Xuan Deng; Kaiyao Huang; Huageng Liang
• Keywords: Zeta potential; Microcystis aeruginosa

Ultrasonic molecular imaging (UMI) technology has attracted increasing interest because of its low cost and capability to evaluate changes rapidly noninvasively at the cellular levels. The key material this is ultrasound-responsive gas vesicles (GVs). GVs synthesized by conventional chemical methods have several limitations, such as high costs, yields, complex production processes. In comparison, biosynthesized advantages stability, a risk toxicity, genetic engineering characterization, easy post modification drug loading potential. However, translational studies their biosynthesis are still in infancy; particular, duration circulatory system essential for usage UMI biomedicine clinic.Here, we report novel cyanobacterium Microcystis, which moderate size, negative zeta potential, rod-like morphology, protein-shelled gas-contained structure. These without any modifications could be detected mice more than 10 hours clinically used ultrasound scanners. can accumulate tumors via enhanced permeation retention (EPR) effect 11 post-injection, lasting least 2 hours, might potential aid tumor diagnosis. Furthermore, pathological hematological study suggested that safe host.We concluded Microcystis systemic evaluation well tumoral diagnosis after intravenous injection.