Effects triggered by platinum nanoparticles on primary keratinocytes

• DOI: 10.2147/ijn.s49612
• Publication Date: 2013-10-16T00:27:44Z
• Authors: Jolanta Jura; Piotr Konieczny; Anna, Grazyna Gor...; Lukasz Skalniak; Joanna Koziel; Francesca, Larese...; Matteo Crosera; Agnieszka Cierniak; Zuba-Surma; Julia Borowczyk; Eliza Laczna; Justyna Drukala; Elzbieta Pyza; Danuta Semik; Olga Woznicka; Andrzej Klein; Radoslaw Szmyd
• Keywords: Primary (astronomy); Platinum nanoparticles

Abstract: The platinum (Pt)-group elements (PGEs) represent a new kind of environmental pollutant and hazard for human health. Since their introduction as vehicle-exhaust catalysts, emissions into the environment have grown considerably compared with low natural concentration in earth crust. PGE from vehicle catalysts can be also form nanometer-sized particles (Pt nanoparticles [PtNPs]). These elements, both metallic or ions solubilized biological media, are now recognized potent allergens sensitizers. Human skin is always exposed to toxic particles; therefore, present study we addressed question whether polyvinylpyrrolidone-coated PtNPs may any negative effects on cells, including predominantly epidermal keratinocytes. In this study, two sizes were used: 5.8 nm 57 nm, concentrations 6.25, 12.5, 25 µg/mL. Both types NPs protected polyvinylpyrrolidone. Primary keratinocytes treated 24 48 hours, then cytotoxicity, genotoxicity, morphology, metabolic activity, changes activation signaling pathways investigated PtNP-treated cells. We found that trigger primary keratinocytes, decreasing cell metabolism, but these no viability migration. Moreover, smaller exhibited more deleterious effect DNA stability than big ones. Analyzing caspases, activity caspase 9 3/7 triggered mainly by NPs. Changes not so significant case larger nanoparticles. Importantly, antibacterial properties, silver (AgNPs). comparison our previous regarding AgNPs biology, do exhibit such they used potential agents, especially treatment Escherichia coli , representing group Gram-negative species. Keywords: nanoparticles, migration, pathways, damage, toxicity