CXCL10 and CXCR3 in the Trigeminal Ganglion Contribute to Trigeminal Neuropathic Pain in Mice

• DOI: 10.2147/jpr.s288292
• Publication Date: 2021-01-12T23:30:40Z
• Authors: Yuan-Yuan Ju; Ming Jiang; Feifei Xu; Dongqin Wang; Bixiao Ding; Ling-Jie Ma; Hao Wu
• Keywords: Trigeminal ganglion; Infraorbital nerve; Allodynia; CXCR3

Trigeminal neuropathic pain is very common clinically, but effective treatments are lacking. Chemokines and their receptors have been implicated in the pathogenesis of chronic pain. This study explored role chemokine CXCL10 its receptor, CXCR3, trigeminal mice.Trigeminal was established by partial infraorbital nerve ligation (pIONL) wild-type Cxcr3-/- mice. Facial mechanical allodynia evaluated behavioral testing. A lentivirus containing Cxcr3 shRNA (LV-Cxcr3 shRNA) microinjected into ganglion (TG) to knock down expression. Quantitative polymerase chain reaction assays immunofluorescence staining were used examine Cxcl10/Cxcr3 mRNA expression protein distribution. Western blotting performed activation extracellular signal-regulated kinase (ERK) AKT TG. Intra-TG injection an inhibitor pain.pIONL induced persistent pain, which alleviated LV-Cxcr3 attenuated pIONL-induced allodynia. Furthermore, pIONL increased CXCR3 major ligand, CXCL10, TG neurons. hypersensitivity mice not also ERK Finally, reduced WT mice.CXCL10 acts on induce neurons contributes maintenance