Exploring the Mechanism of Immediate Analgesia Induced by Tuina Intervention on Minor Chronic Constriction Injury in Rats Using LC-MS

• DOI: 10.2147/jpr.s438682
• Publication Date: 2024-01-24T18:40:59Z
• Authors: - Sachula; Zhenjie Yang; Tianyuan Yu; Jinping Chen; Runlong Zhang; Yingqi Zhang; Jiayue Liu; Hanyu Zhang; Jiawei Sun
• Keywords: KEGG

Purpose: This study aimed to investigate changes in metabolomic expression the spinal dorsal horn (SDH) and thalamus during a Tuina session, aiming elucidate mechanism of immediate analgesia. Methods: The rats were randomly divided into three groups: Sham group, Model group. A minor chronic constriction injury (minor CCI) model was established both group therapeutic effect determined using mechanical withdrawal threshold (MWT) thermal latency (TWL) tests. Differential metabolites SDH detected liquid chromatography-tandem mass spectrometry (LC-MS/MS). Bioinformatic analysis performed CV, PCA, Venn, KEGG. Results: MWT TWL after instant intervention significant. significantly better compared In Veen analysis, it found that instantly regulates 10 differential 5 thalamus. KEGG enrichment we enriched 43 pathways 70 SDH. Conclusion: therapy may have analgesic effects by metabolizing neurotransmitters such as 2-Picolinic Acid, 5-Hydroxy-Tryptophan Glutathione Betaine-aldehyde-chloride Leucine Lysine Methionine Sarcosine Succinic Acid Histidine Acetylcholine 5-Hydroxyindoleacetic through cAMP pathway. It also affects neurodegeneration-multiple diseases, butanoate metabolism, tyrosine metabolism. Keywords: neuropathic pain, tuina, neurotransmitters, thalamus, horn, LC-MS