Efficiency of DPPH and FRAP assays for estimating antioxidant activity and separation of organic acids and phenolic compounds by liquid chromatography in fresh-cut nectarine

• DOI: 10.21475/ajcs.19.13.07.p1368
• Publication Date: 2019-09-11T05:43:43Z
• Authors: Luciana da Silva ...; Giuseppina Pace P...; Francisco Artés; Manoel Euzébio de...; Luís de Souza Fre...; Hanna Ibiapina de...; Núbia de Fátima A...; Márcio Roberto da...
• Keywords: Flavonols; Chlorogenic Acid; Ellagic Acid; Protocatechuic acid

Nectarine contain relevant amounts of antioxidants, including hydroxycinnamic acids, flavan-3-ols, flavonols, anthocyanins, procyanidins, and carotenoids. The aim this research was to compare the efficiency DPPH FRAP assays estimate antioxidant activities contents in nectarine minimally processed. Moreover, separation organic phenolic compounds carotenoids were done by HPLC. fruits ‘R48’ cv. hand harvested, with 80% their reddish coloration. following treatments applied: Control; 1% citric acid + calcium chloride as anti-browning (AB) agent; 4 KJ m-2 UV-C radiation; 8 UV-C; 12 ; AB  95 kPa O2 (High O2); High O2; kJ O2. We determined activity DPPH; FRAP; total phenols HPLC-DAD analyses. In general showed higher concentration comparison processed nectarines. There clear trend content fresh-cut treatment m-2UV-C 22 studied quantified HPLC, being detected: Hydroxycinnamates, anthocyanins. These results allow conclude that most abundant found study chlorogenic acid, caffeic ellagic HPLC chromatograms recorded at 280 nm, quercentin 3-galactoside, quercetin 3- Xyloside 340nm Cyanidin glucoside 510nm.