Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection

• DOI: 10.21577/0103-5053.20160301
• Publication Date: 2016-11-17T12:45:25Z
• Authors: Sivoney Souza; Sergio Kogikoski Jr.; Emerson Silva; Wendel Alves
• Keywords: immunosensor; peptide hydrogels; diphenylalanine; ELISA; Leishmaniasis; 01 natural sciences; 0104 chemical sciences

Hydrogels based on peptide nanostructures are biological entities that can be applied in a wide range of applications, such as scaffolds for tissue engineering, drug delivery, and biosensors.The aim this research was to study hydrogels N-(9-fluorenylmethoxycarbonyl)-L,L-diphenylalanine (Fmoc-FF) two different media: water phosphate buffer.These were used encapsulating Leishmania infantun chagasi soluble proteins.The structure the matrices investigated detail through scanning transmission electron microscopy, small angle X-ray scattering (SAXS).The mechanical behavior assessed rheology assays, demonstrating both physical chemical stability hydrogel scaffolds.The immunogenicity immobilized antigens studied using enzyme-linked immunosorbent assay (ELISA) detection after reaction with positive negative dog sera infantum chagasi.The efficient encapsulate antigens, promote development novel devices requires storage biomolecules under moist environmental conditions.