Promoting of abiotic stress–induced resistance using Poly-β-Hydroxybutyrate (PHB) by Rhizobium phaseoli in common bean plants.

• DOI: 10.21608/ajs.2021.64758.1342
• Publication Date: 2021-06-01T06:24:29Z
• Authors: Shawky Selim; M. ElHaddad; Mahmoud Nassef; Wafaa Radwan; Samah Abu-Hussien
• Keywords: response surface methodology; 2. Zero hunger; abiotic stress; S; rhizobium phaseoli; Agriculture; drought; 15. Life on land; poly-β-hydroxybutyrate (phb); 6. Clean water; salinity

In total, 50 Rhizobium isolates were isolated from the mature root nodules of common beans plants (Phaseolus vulgaris) grown in different nine governorates Egypt.PHB was optimized by identified strain using response surface methodology.A total 11 parameters (pH, incubation period, inoculum size, temperature, agitation speed, mannitol, sucrose, yeast extract, glycine, K2HPO4, and MgSO4) analyzed for their significant effects on PHB production Plackett-Burman design (PBD).Sucrose, MgSO4 main factors affecting accumulation.Central composite (CCD) methodology used to determine optimum levels selected factors.Rhizobium phaseoli reached maximum (4.997 g/L) at run 36 presence 25 g/L 0.0 0.87 0.3 MgSO4, 5% inoculation size.Invitro experiments carried out test effect stress conditions (pH: 6-11, temperature: 5°C-50°C, salinity: 0.01%-7%, drought: 0%-5% w/v) growth phaseoli.The results showed that can withstand 3% -5% NaCl, high temperature 30°C-45°C, alkalinity pH value 8 -10, drought w/v polyethylene glycol with loss 50% when modified medium 75% basal one.In vivo done study bean plants.In general, all treatments superior medium.Also, they tolerance conditions.