Convergent Replication and Mobilization Mechanism of Staphylococcus Pathogenicity Islands (SaPIbov5) by Interfering with Bacteriophage 12 Production Models

• DOI: 10.21608/ejbo.2023.210573.2330
• Publication Date: 2023-08-15T05:47:28Z
• Authors: Tamer Bouback; Faisal Al-Sarraj; Ibrahim Alotibi; Raed Albiheyri; Majed Al-Zahrani; Mashail Alghamdi; Nada Nass; Bayan H. Sajer; Atif Bamagoos; Sheren Azhari; Reem M. Farsi
• Keywords: Replication; Mobilization; Pathogenicity island

S TAPHYLOCOCCUS aureus can enter the bloodstream, leading to health complications such as sepsis, arthritis, endocarditis, and pneumonia.This study screened for impact of six replication genes cos phage 12 (φ12) on evolution transfer S. pathogenicity islands (SaPIbov5).An overnight culture strain RN4220 diluted with fresh TSB was used in bacteriophage φ12 titering assay.Phage-point mutagenesis achieved using pMAD vector, which facilitates homologous recombination a two-step process.Finally, transduction SaPI assays also utilized RN4220, then mixed CaCl 2 TSB.The showed that ORF11, ORF12, VirE mutants did not lyse or produce particles after into recipient strains.While ORF26, ORF10, ORF04 generated detectable plaques, mutations may have an effect packaging tittering.For instance, complemented mutant strains had fully restored titers.The experiment found phages facilitate inter intra-generically cosSaPIs.It established transduces SaPIbov5.However, VirE, ORF11 show mobilization because their effects infective lack replication.The partly titers when they were expressed donor strains.Mutations 0RF10, ORFO4 decrease φ12's ability SaPIbov5.Generally, this new mechanism genes.While affect are necessary biology