Fluoride effect on the process of alveolar bone repair in rats: evaluation of activity of MMP-2 and 9

• DOI: 10.21726/rsbo.v9i3.994
• Publication Date: 2022-08-05T12:58:27Z
• Authors: Mileni da Silva ...; Gisele Miyamura ...; Lucas Makoto Shi...; Flávia Godoy Iano; Marcela Mitsuko ...; Tânia Mary Cestari; Marília Afonso R...; Rodrigo Cardoso ...
• Keywords: Zymography; Matrix (chemical analysis); Alveolar process; Sodium fluoride

The aim of this study was to evaluate comparatively the effect fluoride (F) on activity matrix metalloproteinases 2 and 9 (MMP-2 MMP-9) involved in process alveolar bone repair. Material methods: This used 4 groups Wistar rats with 80 days life (n = 160) which received drinking water containing different doses (NaF): 5, 15, 50 ppm deionized (control) throughout experiment. These animals had their right upper incisors extracted. After extraction, were euthanized at 7, 14, 21 30 hemi-maxillae collected for microscopic analysis (Hematoxylin Eosin immunohistochemistry zymography 9). Results: Microscopically repair similar all groups, being noted only a delay blood clot resorption formation group F. expression MMP-9 showed differences between during initial (7 days). However, no significant treated control groups. Conclusion: Ours results suggest an MMPs period could be associated remission Further studies are needed establish relationship clot, involvement its regulators/tissue inhibitors.