Enhanced Wound Healing with Rhodiola rosea Phytosomal Gel: Extraction, Formulation, and In vivo Evaluation

• DOI: 10.2174/0113816128359150250211053431
• Publication Date: 2025-03-21T07:55:34Z
• Authors: Anandarajagopal K...; Abdullah Khan; Mahibub Mahamadsa...; Syed Atif Abbas; Chiau Ming Long; Tazneem B.; Ching Siang Tan; Abubakar Salam Ba...; Abdul Razak T. K.; Roshan S

Background: This study explores the formulation and effectiveness of a Rhodiola rosea phytosomal gel for enhancing wound healing.

Object: The ethanolic extract of Rhodiola rosea roots, rich in bioactive compounds like alkaloids and flavonoids, was optimized into a phytosomal complex to improve absorption and dermal retention.

Method: Characterization through GC-MS revealed compounds, such as 2-Heptadecenal and Bicyclo[ 4.1.0]Heptane and 7-Pentyl. FTIR confirmed the successful encapsulation within the phospholipid bilayer, while SEM showed smooth, spherical particles.

Result: The Box-Behnken design optimized formulation parameters, achieving high yield (92.64%), small particle size (355 nm), and high entrapment efficiency (93.98%). In vitro release studies displayed a consistent release profile, aligning with Zero-order and Hixson-Crowell models. In vivo evaluation on Wistar rats showed that the phytosomal gel significantly enhanced wound healing, achieving 98.16% wound reduction by day 14, compared to 95.17% for R. rosea extract and 97.13% for standard treatment. Histopathological analysis demonstrated complete tissue regeneration and well-organized collagen fibers in the phytosomal gel group.

Conclusion: This research highlights the potential of Rhodiola rosea phytosomal gel as an effective wound healing therapy, with future studies suggested for extended stability tests and human skin permeation studies.