CD133+ Glioblastoma Stem-Like Cells Induce Vascular Mimicry in Vivo

• DOI: 10.2174/156720211796558023
• Publication Date: 2011-08-01T06:45:39Z
• Authors: Ming-Tsang Chiao; Yi-Chin Yang; Wen-Yu Cheng; Chiung-Chyi Shen; Jiunn-Liang Ko
• Keywords: Vasculogenic mimicry; CD31; Vasculogenesis; Brachyury

Glioblastoma is one of the most angiogenic malignancy, neoplastic vessels which are likely to arise by angiogenesis and vasculogenesis. An alternative mechanism tumor vasculature described, termed vasculogenic mimicry, highly aggressive cells can form vessel-like structures themselves, virtue their high cellular plasticity. Evidence suggests that cancer stem acquire a multi-potent plastic phenotype show potential. In this study, we report glioblastoma stem-like (GSCs) mimicry in xenografts express pro-vascular molecules. We isolated GSCs from resected human tissues demonstrated stemness, differentiation, vivo tumor-initiating Through limiting dilution assay, CD133+ (CD133(+)-GSC) CD133- (CD133(-)-GSC) subpopulation were obtained. Orthotopic xenotransplantation study revealed these two subpopulations shared similar efficacy formation but showed distinct intratumor vasculature. comparison with CD133(-)-GSC, vascularized anaplastic tumor, mimicking was found CD133(+)-GSC-derived xenografts. Subsets CD133(+)-GSC not CD133(-)-GSC capable vascular smooth muscle-like cell vitro vivo. xenografts, endothelium-associated CD31 gene detected implanted exclusively dispersed within tissues. Although detailed action mechanisms required further investigation, capacity brain The results expression molecules differentiation vascular-like suggest may contribute provide blood supply for cells.