Amentoflavone Induces Cell-cycle Arrest, Apoptosis, and Invasion Inhibition in Non-small Cell Lung Cancer Cells

• DOI: 10.21873/anticanres.14893
• Publication Date: 2021-03-04T06:40:17Z
• Authors: WEI-TING CHEN; CHENG-HSIEN CHEN; HUNG-TAI SU; PO-FU YUEH; FEI-TING HSU; I-TSANG CHIANG
• Keywords: Amentoflavone

Background/Aim: Amentoflavone, an effective compound derived from medicinal plants, has been shown to boost therapeutic efficacy of chemotherapy in non-small cell lung cancer (NSCLC). However, anti-NSCLC effect amentoflavone is ambiguous. The major purpose the present study was verify inhibitory effects NSCLC cells. Materials and Methods: on growth invasion CL-1-5-F4 cells were evaluated by viability assay, flow cytometry, colony formation nuclear factor-kappa B (NF-κB) reporter gene immunofluorescence staining, transwell invasion, western blot assay. Results: Amentoflavone effectively induced inhibition, G1 cell-cycle arrest, apoptosis, suppression invasion. Furthermore, not only triggered expression p27, cleaved caspase-3, -8 also reduced NF-κB signaling, protein levels matrix metalloproteinase (MMP)-2, -9, Cyclin-D1, vascular endothelial factor (VEGF). Conclusion: Cell-cycle apoptosis induction, signaling inhibition are associated with amentoflavone-inhibited