Dual Effects of Bilirubin on the Proliferation of Rat Renal NRK52E Cells and its Association with Gap Junctions

• DOI: 10.2203/dose-response.12-003.hei
• Publication Date: 2012-07-31T05:12:20Z
• Authors: Yanling Wang; Qiongfang Zhu; Chenfang Luo; Ailan Zhang; Ziqing Hei; Guangjie Su; Zhengyuan Xia; Michael G. Irwin
• Keywords: Viability assay

Objective The effect of bilirubin on renal pathophysiology is controversial. This study aimed to observe the effects proliferation normal rat tubular epithelial cell line (NRK52E) and its potential interplay with gap junction function. Methods Cultured NRK52E cells, seeded respectively at high- or low- densities, were treated varying concentrations for 24 hours. Cell injury was assessed by measuring viability proliferation, function Parachute dye-coupling assay. Connexin 43 protein Western blotting. Results At doses from 17.1 513μmol/L, dose-dependently enhanced colony-formation rates when cells either densities (all p<0.05 vs. solvent group) accompanied intercellular fluorescence transmission increased Cx43 expression in high-density cells. However, above BR gradually reversed concentration 684 1026μmol/L. In inhibitor 12-O-tetradecanoylphorbol 13-acetate attenuated bilirubin-induced enhancement transmission. presence high (1026μmol/L), activation retinoid acid decreased rates. Conclusion Bilirubin can confer biphasic potentially differentially affecting functions.