Screening of Two Neighboring CFTR Mutations in Iranian Infertile Men with Non-Obstructive Azoospermia.

• DOI: 10.22074/ijfs.2016.4593
• Publication Date: 2017-01-04
• Authors: Somaye Heidari; Zohreh Hojati; Majid Motovali-Bashi
• Keywords: Obstructive azoospermia; ΔF508

The genetic association between cystic fibrosis transmembrane conductance regulator (CFTR) gene mutations and male infertility due to congenital bilateral absence of vas deferens (CBAVD) is well established. Mutant CFTR, however may also be involved in the etiology non-CBAVD cases. present study was conducted estimate frequency ∆I507 ∆F508 CFTR Iranian infertile males. We undertook first these non-obstructive azoospermia Iran. In this case-control study, 100 fertile healthy fathers azoospermia's men were recruited from Isfahan Infertility Center (IIC) Sari Saint Mary's Center, 2008 2009. Screening F508del I507del carried out by multiplex-ARMS-PCR. Significance differences mutation frequencies patient control groups assessed Fisher's exact test. ΔF508 detected three patients. However there are no significant found presence mutated allele [OR=9.2 (allele-based) 7.2 (individual-based), P=0.179]. None samples ΔI507 mutation. Altogether, we show that neither nor population males with azoospermia.