Isolation, identification, biochemical properties and potential application of an organic solvent-tolerant lipase from Pseudomonas sp. strain NEB-1

• DOI: 10.22099/ijsts.2014.2264
• Publication Date: 2014-04-16
• Authors: M. Abdollahi; Hamid Reza Karbal...
• Keywords: Strain (injury)

Using enrichment procedures, 45 organic solvent-tolerant lipase producer bacterial strains were screened from areas contaminated by oil and solvents. Among the strains, 15 isolates exhibited extreme stability toward solvents high lipolytic activity. The NEB-1 isolate which was later identified as Pseudomonas sp. strain biochemical tests 16S rDNA gene sequence analysis selected based on extremely tolerance to maximum production. Biochemical studies revealed that crude stable at temperatures between 20 oC 60 pH ranges of 4 11 for 1 h. Optimum temperature enzyme be 9.5 70 oC, respectively. showed remarkable in presence different with a broad range hydrophobicity characteristics. solvent an attractive potency application biocatalysis non-aqueous systems biodiesel