Concentration and Integrity Indexes of Urine Cell-Free DNA as Promising Biomarkers for Early Lung Cancer Diagnosis

• DOI: 10.2217/pme-2020-0019
• Publication Date: 2021-02-10T12:44:31Z
• Authors: Sai Ren; Xiaodong Ren; Haiqin Guo; Lan Liang; Kun Wei; Lifang Guo; Xuemei Qu; Xiaotian Dai; Qing Huang
• Keywords: Cell-free fetal DNA; Liquid biopsy

Aim: To explore the role of urine cell-free DNA (ucfDNA) concentration and integrity indexes as potential biomarkers for lung cancer diagnosis. Materials & methods: Quantitative real-time PCR targeting Arthrobacter luteus (ALU) repeats at three size fragments (ALU-60, 115 247 bp) was performed in 55 patients 35 healthy individuals. Results: ucfDNA were significantly higher than controls. The area under receiver operating characteristic curve differentiating with stage I/II from controls by ALU 0.856, 0.909 0.932, respectively. In addition, lymph node metastasis non-metastatic. Conclusion: could serve promising