Haemocompatibility evaluation of DLC- and SiC-coated surfaces

• DOI: 10.22203/ecm.v005a02
• Publication Date: 2016-11-21T07:06:25Z
• Authors: N Nurdin; P François; Y Mugnier; J Krumeich; M Moret; B-O Aronsson; P Descouts
• Keywords: Blood Platelets; 570; Thrombin generation; Diamond-like carbon coating; Surface Properties; Complement convertase attachment; Carbon Compounds, Inorganic; plasma depo- sition; 610; [PHYS.MECA.MSMECA] Physics [physics]/Mechanics [physics]/Materials and structures in mechanics [physics.class-ph]; Biocompatible Materials; [SPI.MECA.MSMECA]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Materials and structures in mechanics [physics.class-ph]; ddc:500.2; Complement C3-C5 Convertases; 02 engineering and technology; info:eu-repo/classification/ddc/500.2; Platelet Adhesiveness; Coated Materials, Biocompatible; info:eu-repo/classification/ddc/616; Materials Testing; Spectroscopy, Fourier Transform Infrared; [SPI.MECA.MSMECA] Engineering Sciences [physics]/Mechanics [physics.med-ph]/Materials and structures in mechanics [physics.class-ph]; Humans; ddc:616; Silicon Compounds; Thrombin; [PHYS.MECA.MSMECA]Physics [physics]/Mechanics [physics]/Materials and structures in mechanics [physics.class-ph]; Platelet Activation; complement convertase attachment; Carbon; complement convertase attachment.; thrombin generation; Enzyme Induction; Microscopy, Electron, Scanning; Plasma deposition; platelet adhesion; Diamond; 0210 nano-technology

Diamond-like carbon (DLC) and silicon carbide (SiC) coatings are attractive because of low friction coefficient, high hardness, chemical inertness smooth finish, which they provide to biomedical devices. Silicon wafers (Si(waf)) silicone rubber (Si(rub)) plates were coated using plasma-enhanced vapour deposition (PE-CVD) techniques. This article describes: 1- the characterization modified surfaces attenuated total reflection-Fourier transform infrared spectroscopy (ATR/FTIR) contact angle measurements, 2- results three in-vitro haemocompatibility assays. Coated compared uncoated materials various substrates such as polymethylmethacrylate (PMMA), polyethylene (LDPE), polydimethylsiloxane (PDMS) medical steel (MS). Thrombin generation, blood platelet adhesion complement convertase activity tests revealed following classification, from most least heamocompatible surface: Si(rub)/ DLC-Si(rub)/ DLC-Si(waf)/ LDPE/ PDMS/ SiC-Si(waf)/ Si(waf)/ PMMA/ MS. The DLC coating delayed clotting time, tended inhibit activation, whereas SiC-coated wafer can be considered thrombogenic. study has taken into account events activation: coagulation, activation inflammation. response those is an indicator in vitro different it allows us select biomaterials for further vivo contacting investigations.