NANOG priming before full reprogramming may generate germ cell tumours

• DOI: 10.22203/ecm.v022a20
• Publication Date: 2016-12-06T10:22:59Z
• Authors: I Grad; Y Hibaoui; M Jaconi; L Chicha; R Bergström-Tengz...; MR Sailani; MF Pelte; S Dahoun; TA Mitsiadis; V Töhönen; S Bouillaguet; SE Antonarakis; J Kere; M Zucchelli; O Hovatta; A Feki
• Keywords: Homeobox protein NANOG; Reprogramming; KLF4; LIN28; Cell potency; Rex1; Germ layer

Reprogramming somatic cells into a pluripotent state brings patient-tailored, ethical controversy-free cellular therapy closer to reality.However, stem and cancer share many common characteristics; therefore, it is crucial be able discriminate between them.We generated two induced cell (iPSC) lines, with NANOG pre-transduction followed by OCT3/4, SOX2, LIN28 overexpression.One of the CHiPS W, showed normal characteristics, while other, A, though expressing pluripotency markers, failed differentiate gave rise germ cell-like tumours in vivo.Comparative genomic hybridisation analysis iPS lines revealed that they were genetically more stable than human embryonic counterparts.This proved predictive for differentiation potential analysed cells.Moreover, A line expressed lower ratio p53/p21 when compared W. pre-induction MYC, KLF4 induction resulted same tumour-inducing phenotype.These results underline importance re-examination role during reprogramming.Moreover, this reprogramming method may provide insights primordial tumour formation transformation.