A bilayered tissue engineered in vitro model simulating the tooth periodontium

• DOI: 10.22203/ecm.v042a17
• Publication Date: 2021-10-11T08:38:22Z
• Authors: A Khadre; ELM Raif; S Junaid; OM Goudouri; W Refaat; A Ramadan; AR Boccaccini; R El-Gendy
• Keywords: Periodontal fiber; Periodontium

Due to the complexity of structure tooth periodontium, regeneration full attachment is not a trivial task. There also gap in models that can represent human vitro and vivo. The aim this study was develop bilayered construct simulated periodontal ligament attached alveolar bone, for purpose tissue investigation physiological orthodontic loading. Two types materials were used construct: sol-gel 60S10Mg derived scaffold, representing hard component commercially available Geistlich Bio-Gide® collagen membrane, soft attachment. Each scaffold dynamically seeded with cells (HPDLCs). Scaffolds either cultured separately, or combined construct, 2 weeks. Characterisation individual scaffolds constructs included biological characterisation (cell viability, scanning electron microscopy confirm cell attachment, gene expression periodontium markers), mechanical constructs. HPDLCs enjoyed biocompatible 3-dimensional environment within components. no drop cellular compared separate scaffolds.