In silico Approaches for the detection of organophosphate pesticides by OPH-Enzyme based biosensors

• DOI: 10.22271/j.ento.2020.v8.i6ab.9101
• Publication Date: 2023-07-06T15:41:00Z
• Authors: V Aruna Devi; M Vasanthi; K Kanagarajadurai
• Keywords: Docking (animal)

Among the various groups of pesticides that are being used world over, p-Nitro phenyl (pNP)-substituent Organophosphates (OPs) acutely toxic and more extensively group pesticides. Organophosphorus hydrolase (OPH) is an enzyme involved in hydrolysis aromatic OPs Thus, OPH extremely attractive biological component biosensors for detection pNP substituent concentration. The difference structural organisation from different microbe will influences catalytic activity these enzymes. So, rationale behind choosing specific microbial as a biocomponent not yet reported. current study has been performed to predict based on sequence analysis well modelling docking studies correlation binding energy with experimentally determined affinities validation approach. Based silico study, two enzymes species predicted show high specificity it may be recommended use them after validating through vitro vivo analysis.