Discovery of a novel recombinant avian orthoreovirus in China

• DOI: 10.22541/au.164865001.11200075/v1
• Publication Date: 2022-03-30T14:20:20Z
• Authors: Tian Yan; Xiaoning Jiang; Hongzhi Wang; Zhonghui Yao; Siming Zhu; YOU XIANG DIAO; YI TANG
• Keywords: Flock; Strain (injury)

In mid-2020, using next-generation sequencing (NGS) technology, we identified a divergent cluster 2 avian orthoreovirus (ARV) variant named PHC-2020-0545, isolated from tendons of 33-day-old broilers with leg swelling in China. Sequence comparison and phylogenetic analysis results revealed that the isolate strain was genetically distinct all known ARV strains M1 M3 genes, σC coding gene quite different grouped into other genotyping cluster. The recombination multiple segmental recombination, intra-segmental accumulation point mutations might contribute to emergence this isolate. infected PHC-2020-0545 showed typical clinical symptoms tarsal joint swelling, mainly affected movement, digestion metabolism broilers. Although mortality caused by very low, viral load detection it had strong replication ability vivo. addition, infection route is related its pathogenicity Therefore, determined field unique novel recombinant strain, which become prevalent Chinese broiler flocks.