Structural Analysis of Experimentally Induced Disc Herniation-Like Changes in the Rat

• DOI: 10.22603/ssrr.2019-0010
• Publication Date: 2019-07-09T22:03:57Z
• Authors: Satoshi Ujigo; Daniel Jonsson; Yalda Bogestål; Joakim Håkansson; Jennifer Rosendahl; Lena Brive; Kjell Olmarker
• Keywords: Intervertebral Disc; Nodule (geology); Scar tissue

A disc herniation has traditionally been considered as tissue that slipped out from an intervertebral disc. However, it was recently suggested the mass is a product of bioactive substances and hernia would more likely be scar than herniated material. In this study, we aimed to analyze structural components experimentally induced herniations compare with nucleus pulposus, in rat.Twenty-eight rats had their L4-5 discs punctured. After three weeks, nodule formed over puncture site, spine musculature, normal pulposus were harvested processed for further analysis.Proteomics analysis demonstrated similar pulposus. Gene expression showed there no resemblance between any tissues when looking at inflammatory genes but that, clear analyzing extracellular matrix-related genes. Analysis GAG polysaccharide size distribution revealed only contained shorter versions, potentially short chain hyaluronic acid known induce responses. The hematoxylin eosin stained sections nodule, tissue, indicated morphology very similar.The after experimental puncture, resembles hernia, tissue. is, therefore, by disc-derived being