Development and validation of SPE-HPLC method for the determination of carbamazepine and its metabolites carbamazepine epoxide and carbamazepine trans-diol in plasma

• DOI: 10.2298/jsc120106084d
• Publication Date: 2012-08-10T13:27:13Z
• Authors: Predrag Dzodic; Ljiljana Zivanovic; Ana Protic; Ivana Ivanovic; Radmila Velickovi...; Mirjana Spasic; Stevo Lukic; Slavoljub Zivanovic
• Keywords: Quantitative Analysis; Solid phase extraction

SPE-HPLC method has been developed and validated for rapid analysis of carbamazepine its two metabolites epoxide trans-diol in human plasma. The was performed using C18 Bakerbond-BDC analytical column (250 mm x 4.6 i.d., particle size 5 ?m). optimal conditions the separation were established with mobile phase acetonitrile - 10 mM phosphate buffer, pH 7.0 (30:70, v/v) at flow rate 1.5 mL min-1, temperature 35?C, UV detection 210 nm. Total run time about 8 minutes. SPE procedure extraction analytes from plasma sample Oasis HLB cartridges subsequently eluate injected into HPLC system analysis. Afterwards, subjected to validation. Linearity obtained over concentration range 0.2-25 ?g/mL carbamazepine, correlation coefficients higher than 0.995. showed good intra-day inter-day precision relative standard deviation below 7.96%, while accuracy ranged 92.09% 108.5% all analytes. Finally, successfully applied samples epileptic patients monotherapy polytherapy. [Acknowledgments. Projekat Ministarstva nauke Republike Srbije, br. OI 172033].