Comparison of Serum High–Molecular Weight (HMW) Adiponectin With Total Adiponectin Concentrations in Type 2 Diabetic Patients With Coronary Artery Disease Using a Novel Enzyme-Linked Immunosorbent Assay to Detect HMW Adiponectin

• DOI: 10.2337/db05-1525
• Publication Date: 2006-06-27T18:39:44Z
• Authors: Yoshimasa Aso; Ruriko Yamamoto; Sadao Wakabayashi; Toshihiko Uchida; Kan Takayanagi; Kohzo Takebayashi; Takehiko Okuno; Teruo Inoue; Koichi Node; Takashi Tobe; Toshihiko Inukai; Yasuko Nakano
• Keywords: Blood Glucose; Male; Coronary Disease; Enzyme-Linked Immunosorbent Assay; Middle Aged; Lipids; 3. Good health; Molecular Weight; Protein Subunits; 03 medical and health sciences; 0302 clinical medicine; Diabetes Mellitus, Type 2; Humans; Female; Adiponectin; Diabetic Angiopathies; Aged

Adiponectin (Acrp30), an adipocyte-derived protein, exists in serum as a trimer, hexamer, and high-molecular weight (HMW) form, including 12-18 subunits. Because HMW adiponectin may be biologically active, we measured it using novel enzyme-linked immunosorbent assay (ELISA) confirmed by gel filtration chromatography that the ELISA detected mainly with subunits, compared total concentration patients type 2 diabetes. We next investigated relationship between coronary artery disease (CAD) 280 consecutive diabetic patients, 59 angiographically CAD. Total was commercially available ELISA. Like adiponectin, correlated positively HDL cholesterol negatively triglyceride, insulin sensitivity, creatinine clearance, circulating inflammatory markers. were significantly higher women than men, HMW-to-total ratio. Serum ratio lower men without CAD (P < 0.05, respectively). In women, ratio, but neither nor tended to when present. conclusion, determination of especially relative is useful for evaluating patients.