No Alterations in the Frequency of FOXP3+ Regulatory T-Cells in Type 1 Diabetes

• DOI: 10.2337/db06-1248
• Publication Date: 2007-02-27T18:14:43Z
• Authors: Todd Brusko; Clive Wasserfall; Kieran McGrail; Richard Schatz; Hilla Lee Viener; Desmond Schatz; Michael Haller; Jennifer Rockell; Peter Gottlieb; Michael Clare-Sal...; Mark Atkinson
• Keywords: Interleukin-7 receptor

Regulatory T-cells (Tregs) play a critical role in maintaining dominant peripheral tolerance. Previous characterizations of Tregs type 1 diabetes have used antibodies against CD4 and alpha-chain the interleukin-2 receptor complex (CD25). This report extends those investigations by addition more lineage-specific marker for Tregs, transcription factor forkhead box P3 (FOXP3), subjects with diabetes, their first-degree relatives, healthy control subjects. With inclusion this marker, two predominant populations CD4(+)CD25(+) were identified: CD4(+)CD25(+)FOXP3(+) as well CD4(+)FOXP3(-) expressing low levels CD25 (CD4(+)CD25(LOW)FOXP3(-)). In all study groups, frequency cells was age independent, whereas CD4(+)CD25(LOW)FOXP3(-) cell frequencies strongly associated age. terms additional markers delineating Treg lineage, FOXP3(+) CD127(-) to CD127(LOW) CD25(+) less restricted expression CD127 expressed across continuum levels. Importantly, no differences observed individuals or at varying degrees risk diabetes. These suggest that altered blood defined FOXP3, are not specifically continue highlight an important variable analysis immune regulation.