Islet Surface Heparinization Prevents the Instant Blood-Mediated Inflammatory Reaction in Islet Transplantation

• DOI: 10.2337/db07-0358
• Publication Date: 2007-07-27T17:34:04Z
• Authors: Sanja Cabric; Javier Sanchez; Torbjörn Lundgren; Aksel Foss; Marie Felldin; Ragnar Källen; Kaija Salmela; Annika Tibell; Gunnar Tufveson; Rolf Larsson; Olle Korsgren; Bo Nilsson
• Keywords: Pancreatic Islets

In clinical islet transplantation, the instant blood-mediated inflammatory reaction (IBMIR) is a major factor contributing to poor initial engraftment of islets. This triggered by tissue and monocyte chemoattractant protein (MCP)-1, expressed transplanted pancreatic islets when come in contact with blood portal vein. All currently identified systemic inhibitors IBMIR are associated significantly increased risk bleeding or other side effects. To avoid treatment, aim present study was render graft biocompatible applying continuous heparin coating surface.A biotin/avidin technique used conjugate preformed complexes surface endothelial-like achieved conjugating barely 40 IU per full-size transplant.Both an vitro loop model allogeneic porcine this provided protection against IBMIR. Culturing heparinized for 24 h did not affect insulin release after glucose challenge, heparin-coated cured diabetic mice manner similar untreated islets.This novel pretreatment procedure prevents intraportal thrombosis efficiently inhibits without increasing and, unlike procedures (e.g., gene therapy), inducing acute chronic toxicity