Regulatory Mechanisms for Adipose Tissue M1 and M2 Macrophages in Diet-Induced Obese Mice

• DOI: 10.2337/db08-1475
• Publication Date: 2009-08-19T03:44:36Z
• Authors: Shiho Fujisaka; Isao Usui; Agussalim Bukhari; Masashi Ikutani; Takeshi Oya; Yukiko Kanatani; Koichi Tsuneyama; Yoshinori Nagai; Kiyoshi Takatsu; Masaharu Urakaze; Masashi Kobayashi; Kazuyuki Tobe
• Keywords: Adipose tissue macrophages; Pioglitazone; CD11c; Monocyte; CD163

OBJECTIVE To characterize the phenotypic changes of adipose tissue macrophages (ATMs) under different conditions insulin sensitivity. RESEARCH DESIGN AND METHODS The number and expressions marker genes for M1 M2 from mouse epididymal fat were analyzed using flow cytometry after mice had been subjected to a high-fat diet (HFD) pioglitazone treatment. RESULTS Most CD11c-positive CD206-positive in clearly separated cytometry. exhibited completely gene expression patterns. Not only numbers ATMs genes, such as tumor necrosis factor-α monocyte chemoattractant protein-1, but also M1-to-M2 ratio increased by an HFD decreased subsequent treatment, suggesting correlation with whole-body We found that was associated upregulated interleukin (IL)-10, anti-inflammatory Th2 cytokine, adipocyte fraction well tissue. systemic overexpression IL-10 adenovirus vector markers CONCLUSIONS constitute subsets macrophages. Insulin resistance is both ratio. might be involved recruitment