Pigment Epithelium–Derived Factor Regulates Lipid Metabolism via Adipose Triglyceride Lipase

• DOI: 10.2337/db10-0845
• Publication Date: 2011-04-05T03:54:47Z
• Authors: Melissa L. Borg; Zane B. Andrews; Elia J. Duh; Rudolf Zechner; Peter J. Meikle; Matthew J. Watt
• Keywords: Adipose triglyceride lipase; Hormone-sensitive lipase; PEDF; Fatty Acid Metabolism

OBJECTIVE Pigment epithelium–derived factor (PEDF) is an adipocyte-secreted involved in the development of insulin resistance obesity. Previous studies have identified PEDF as a regulator triacylglycerol metabolism liver that may act through adipose triglyceride lipase (ATGL). We used ATGL−/− mice to determine role regulating lipid and glucose metabolism. RESEARCH DESIGN AND METHODS Recombinant was administered wild-type mice, whole-body energy studied by indirect calorimetry. Adipose tissue lipolysis skeletal muscle fatty acid determined isolated preparations. Muscle lipids were assessed electrospray ionization–tandem mass spectrometry. Whole-body sensitivity uptake assessed. RESULTS impaired capacity adjust substrate selection, resulting delayed diurnal decline respiratory exchange ratio, suppressed daily oxidation. enhanced adipocyte activity muscle. storage unaffected, whereas oxidation impaired. These changes abrogated not attributable hypothalamic actions. also refractory PEDF-mediated resistance, but this related species CONCLUSIONS The results are first direct demonstration 1) influences systemic promoting ATGL-dependent manner reducing 2) ATGL required for negative effects on action.