Central Leptin Signaling Is Required to Normalize Myocardial Fatty Acid Oxidation Rates in Caloric-Restricted ob/ob Mice

• DOI: 10.2337/db10-1106
• Publication Date: 2011-03-26T03:56:15Z
• Authors: Crystal Sloan; Joseph Tuinei; Katherine Nemetz; Jonathan Frandsen; Jamie Soto; Noah Wride; Tomoki Sempokuya; Luis Alegria; Heiko Bugger; E. Dale Abel
• Keywords: Intraperitoneal injection; Fatty Acid Metabolism

ob/ob and db/db mice manifest myocardial hypertrophy, insulin resistance, altered substrate utilization, mitochondrial dysfunction, lipid accumulation. This study was designed to determine the contribution of central peripheral leptin signaling metabolism function in absence diabetes morbid obesity.Male (aged 4 weeks) were caloric restricted by pairfeeding a leptin-treated group. In addition determining glucose tolerance circulating concentrations, determined saponin-permeabilized cardiac fibers. Second, experiments performed whether treatment intraperitoneal injection or intracerebroventricular infusion could normalize palmitate oxidation caloric-restricted mouse hearts.Despite normalizing body weight tolerance, fat mass levels remained increased animals. Palmitate elevated hearts normalized leptin. Intraperitoneal also free fatty acid levels, gene expression, sensitivity.These data suggest that impaired hypothalamic is sufficient increase increasing delivery substrates peroxisome proliferator-activated receptor-α ligands heart.