High-Glucose Environment Enhanced Oxidative Stress and Increased Interleukin-8 Secretion From Keratinocytes

• DOI: 10.2337/db12-1714
• Publication Date: 2013-02-20T02:39:06Z
• Authors: Cheng-Che E. Lan; Ching-Shuang Wu; Shu-Mei Huang; I-Hui Wu; Gwo-Shing Chen
• Keywords: Infiltration (HVAC); Interleukin 8

Impaired wound healing frequently occurs in patients with diabetes. Interleukin (IL)-8 production by keratinocyte is responsible for recruiting neutrophils during healing. Intense inflammation associated diabetic wounds, while reduction of neutrophil infiltration enhanced We hypothesized that increased recruitment keratinocytes may contribute to the delayed wounds. Using cultured human and a rat model, current study shows high-glucose environment IL-8 via epidermal growth factor receptor (EGFR)-extracellular signal-regulated kinase (ERK) pathway reactive oxygen species (ROS)-dependent manner keratinocytes. In addition, skin showed EGFR, ERK, expression compared control rats. The dermal wound, as represented myeloperoxidase level, was also significantly higher Treating rats dapsone, an agent known inhibit function, improved conclusion, are due elevated ROS level contributed impaired skin. Targeting these dysfunctions present novel therapeutic approaches.