Accumulation of Endogenous Asymmetric Dimethylarginine Contributes to Diabetic Cardiomyopathy via Endoplasmic Reticulum Stress in Diabetic Rats

• DOI: 10.2337/db18-2182-pub
• Publication Date: 2018-06-22T18:57:33Z
• Authors: YAN XIONG; YULIAN HE; WEI-JIN FANG; YANPING LEI; YUAN LIN; ZHI-MIN HE
• Keywords: Diabetic Cardiomyopathy; Asymmetric dimethylarginine

Diabetic cardiomyopathy is a major cause of morbidity and mortality among diabetic patients. Endoplasmic reticulum (ER) stress has been involved in cardiomyopathy. Accumulation endogenous nitric oxide synthase (NOS) inhibitor asymmetric dimethylarginine (ADMA) associated with cardiovascular complications. This study was to determine whether ADMA accumulation contributes its mechanism related ER streptozotocin-induced rats cultured rat cardiomyocytes. Cardiac function measured by M-echocardiography. reflected transcription glucose-regulated protein78, phosphorylation eukaryotic initiation factor 2α kinase, splicing X box-binding protein-1 mRNA expression C/EBP homologues protein. Changes signal pathway were evaluated contents, NOS dimethylaminohydrolase activities, as well their protein expression. The levels serum myocardium significantly elevated compared control rats. elevation left ventricular diastolic systolic dysfunction comparison Furthermore, not only coupled the disorder pathway, but also tied oxidative Treatment antioxidant N-acetylcysteine could reduce stress, ameliorate cardiac Similar results observed exogenous inhibitor-treated normal ADMA-incubated These indicate that plays pivotal role development cardiomyopathy, underlying close stress. Disclosure Y. Xiong: None. He: W. Fang: Lei: Lin: Z.